PEGASUS® GC-HRT+ 4D

High Resolution TOFMS

The Pegasus GC-HRT+ 4D combines the highest performance GC×GC and TOFMS tools on the market with High Resolution Deconvolution® (HRD®). Ideal for the most complex samples, users are able to find more analytes than ever before and confidently identify unknown components. ChromaTOF® brand software, designed specifically for HRT+ instrumentation and GC×GC support, adds advanced qualitative and quantitative capabilities to an all-in-one user-friendly data system.

PEGASUS® GC-HRT+ 4D Details

Available Models

  • Pegasus GC-HRT+ 4D with Cryogen-Free Modulator

Available Options

  • L-PAL3 Automated Sample Injector

Features

  • Mass accuracies of 1 ppm and peak capacity potential at least two times greater than any mass spectrometer currently available on the marketplace

  • Encoded Frequent Pushing® (EFP®) contributes to a significant increase in sensitivity, expanded dynamic range, wide mass range, and much more

  • Folded Flight Path (FFP) technology provides high resolution, enhanced sensitivity, and higher orders of TOF focusing

  • KADAS® – our patented ion statistics-based data acquisition system, dramatically reduces noise, ensures mass accuracy, and reduces file size

  • Integrated NIST and Accurate Mass Libraries compare accurate mass new sample data against an accurate mass library data set.

  • Available with either Electron Ionization (EI) source for comparison with classic library spectra, or Chemical Ionization (CI) source for preservation of the molecular ion

Theory of Operation

The Pegasus GC-HRT+ 4D provides the capability to obtain high-resolution mass spectral data using comprehensive two-dimensional gas chromatography (GC×GC). GC×GC provides high peak capacity by trapping compounds eluting from the first chromatographic column and then systematically injecting them into the second column to obtain the second separation. The separation on the second chromatographic phase is completed in seconds, resulting in very narrow chromatographic peaks (<200 milliseconds).

To obtain sufficient data to define a 2D chromatographic peak, acquisition rates of at least 100 spectra/second are required. With the Pegasus GC-HRT+ 4D, data acquisition rates of up to 200 spectra/second can be utilized, giving sufficient data density to obtain spectral deconvolution of the chromatographic peaks. Combined, LECO’s GC×GC chromatography and the Pegasus GC-HRT+ provide the ultimate solution in complex sample characterization.

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TESTIMONIALS

That‘s what our users say:

Helmholtz Zentrum München (HMGU) Department “Comprehensive Molecular Analytics“, GERMANY

„This combination of ultrahigh chromatographic separation with high mass resolution might be still unique for a commercial
available end user system.“

Prof. Ralf Zimmermann

Food Chemistry Institute Cologne, Italy

“Pegasus BT is the perfect choice for identifying well-known metabolites at low concentration level in our sample, and also discovering new compounds. The most surprising advantage of the BT system is the ion source robustness! We usually work with dirty and complex samples, but we have never observed drop of sensitivity during our analyses.This reduces the time and cost of our analyses!”

Prof. Antonio Randazzon

Università degli Studi del Piemonte Orientale, Italy

„With the Pegasus BT 4D performing untargeted analysis today is very easy, thanks to the high sensitivity of the instrument, we are able to mine and analyze any type of sample! A combination of very good sensitivity, quality to price ratio and high flexibility make the Pegasus BT 4D system perfect for analysis of every sample”

Dott. Marcello Manfredi

Czech Academy of Science, Tschechische Republik

“Compared to GC equipped with a single column, the Pegasus 4D enabled me to separate unbelievable number of analytes! Tens of thousands of separated peaks in one run was not an exception. But where the separation was not perfect, ChromaTOF software and its deconvolution algorithm did the job. Such excellent ‘separating power’ helped me discover new types of plant PCB metabolites.“

Ing. Jan Rezek, Ph. D.

TURKU Metabolomics Facility, Finland

“We use the LECO Pegasus BT instrument for the quantification of small polar metabolites. We developed a hybrid method where we quantitate 51 metabolites with standard curves and screen for any other small polar metabolites in a wide range of biological samples. This assay has a great dynamic range thanks to the sensitivity of the BT. The StayClean® ion source allows us to run hundreds of samples in a batch and be confi dent that the ion source will remain clean even with dirty samples such as plasma.“

Alex Dickens

SGS Institut Fresenius GmbH Berlin, Germany

“We at SGS MOSH/MOAH group are using the GC × GC-TOFMS from LECO to separate the highly complex fractions of MOSH/MOAH contaminates in food. When you want to identify special patterns of contamination species amongst other analytics (e. g carotenoids) you need a high separation power in combination with a fast screening MS. This is provided by the LECO TOF. Actually we have a new project together with LECO and another company to quantify MOSH/MOAH fractions. The complexity of the instrumentation is high, but with the support of the application team from LECO we reach our goals.”

Silvia Aguiló Losa

ALS Enviromental, UK

“I believe this technology and methodology will profoundly change water analysis realising major benefi ts for both laboratories and their customers.“

John Quick, Principal Scientist

LECO INSTRUMENTS UK LTD.
Unit 7, Rhino Court, Station View
Bramhall More Lane, Hazel Grove
Stockport SK7 5ER
ENGLAND

Phone: +44 161 487 5900
Email: general_uk@leco.com

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