Comprehensive Two-Dimensional Gas Chromatography
LECO’s QuadJet™ SD is the ideal solution for samples which arrive in your lab. By combining the sensitivity of Flame Ionization Detection (FID) with the increased chromatographic resolution of comprehensive two-dimensional gas chromatography (GC×GC), the QuadJet SD delivers a better measure of the actual components of your sample than a straight GC analysis. No other system available on the market can deliver the same reduction in noise and error as the QuadJet SD.
Theory of Operation
When performing GC-FID analysis, most laboratories quantify compounds using the area of the FID signal. Real world samples are rarely clean and straightforward. For example, a diesel sample can have thousands of different compounds in a single injection. The more compounds in a sample, the higher the chance of coelution; this coelution will increase the FID signal, thus creating a high bias error in the measurement. With GC×GC performing two separate chromatographic separations in one experiment, the accuracy of the FID measurement is maintained.
Due to the orthogonal nature of the separation mechanism in GCxGC, coeluting compounds are easily pulled apart in a single run. True GC×GC instruments, such as the LECO QuadJet SD, improve upon simply running the same sample through two different columns by incorporating a modulation device. Peaks eluting from the primary column are quantitatively segmented into smaller sections prior to release onto the second column. This segmenting, along with the cryo-focusing of the modulator, can deliver peak widths as narrow as 50 ms to the second column.
There is no better-performing modulator for GC×GC than thermal modulation, and not even other thermal modulators can match up to the ruggedness, reliability, and performance of LECO’s GC×GC modulator. With LECO’s QuadJet SD, modulation is accomplished via a dual-stage, quad-jet thermal modulator positioned between the two columns. LECO’s thermal modulator allows for on-column cryo-focusing within the GC×GC system, providing increased peak detectability and increased separation of coeluting compounds for an order-of-magnitude increase in signal-to-noise.
LECO’s Consumable-Free Modulator (which does not utilize Liquid Nitrogen) is an available option, saving time and money without sacrificing performance when modulating moderately volatile compounds.
LECO’s QuadJet SD is designed to give you the upper hand in laboratory productivity. Its ease-of-use and industry’s best secondary oven for the secondary column allows for improved analytical results. When you take the step to the next dimension in gas chromatography, make sure you’re taking the step that will let you see what you’ve been missing.
QuadJet™ SD Details
- QuadJet™ SD With LN2 Cooled Thermal Modulator
- QuadJet™ SD With Consumable-Free Modulator
- Optional Inlets Available (Including S/SL, PTV, And MMI)
QuadJet™ SD Brochure
That‘s what our users say:
Helmholtz Zentrum München (HMGU) Department “Comprehensive Molecular Analytics“, GERMANY
„This combination of ultrahigh chromatographic separation with high mass resolution might be still unique for a commercial
available end user system.“
Food Chemistry Institute Cologne, Italy
“Pegasus BT is the perfect choice for identifying well-known metabolites at low concentration level in our sample, and also discovering new compounds. The most surprising advantage of the BT system is the ion source robustness! We usually work with dirty and complex samples, but we have never observed drop of sensitivity during our analyses.This reduces the time and cost of our analyses!”
Università degli Studi del Piemonte Orientale, Italy
„With the Pegasus BT 4D performing untargeted analysis today is very easy, thanks to the high sensitivity of the instrument, we are able to mine and analyze any type of sample! A combination of very good sensitivity, quality to price ratio and high flexibility make the Pegasus BT 4D system perfect for analysis of every sample”
Czech Academy of Science, Tschechische Republik
“Compared to GC equipped with a single column, the Pegasus 4D enabled me to separate unbelievable number of analytes! Tens of thousands of separated peaks in one run was not an exception. But where the separation was not perfect, ChromaTOF software and its deconvolution algorithm did the job. Such excellent ‘separating power’ helped me discover new types of plant PCB metabolites.“
TURKU Metabolomics Facility, Finland
“We use the LECO Pegasus BT instrument for the quantiﬁcation of small polar metabolites. We developed a hybrid method where we quantitate 51 metabolites with standard curves and screen for any other small polar metabolites in a wide range of biological samples. This assay has a great dynamic range thanks to the sensitivity of the BT. The StayClean® ion source allows us to run hundreds of samples in a batch and be conﬁ dent that the ion source will remain clean even with dirty samples such as plasma.“
SGS Institut Fresenius GmbH Berlin, Germany
“We at SGS MOSH/MOAH group are using the GC × GC-TOFMS from LECO to separate the highly complex fractions of MOSH/MOAH contaminates in food. When you want to identify special patterns of contamination species amongst other analytics (e. g carotenoids) you need a high separation power in combination with a fast screening MS. This is provided by the LECO TOF. Actually we have a new project together with LECO and another company to quantify MOSH/MOAH fractions. The complexity of the instrumentation is high, but with the support of the application team from LECO we reach our goals.”
ALS Enviromental, UK
“I believe this technology and methodology will profoundly change water analysis realising major beneﬁ ts for both laboratories and their customers.“
LECO INSTRUMENTS UK LTD.
Unit 7, Rhino Court, Station View
Bramhall More Lane, Hazel Grove
Stockport SK7 5ER
Phone: +44 161 487 5900